Slide of human sperm
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Human sperm slide, smear
Prepared Slides, Human Sperm Smear, Each - American Scientific
Sperm are much tinier than red blood cells. Look for the filament tail on individual sperm. Read More. Our products are durable, reliable, and affordable to take you from the field to the lab to the kitchen.
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Preparation of Sperm Slide
Remove the supernatant and resuspend the pellet in 10 mL of 1X PBS for a new wash by centrifugation 5 min at ,. Using a Pasteur pipet, drop one droplet of the sperm suspension on a clean microscope slide from a height of approx 10 cm. The slide is air-dried and checked under the light microscope x10 and x40 to ensure that both the cell concentration and the spreading are optimum see Note 2. Using a diamond marker, draw a circle on the underside of the slide to mark where the spermatozoa are.
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